It is known that human saliva includes various kinds of salivary proteins that show genetic polymorphism. With respect to salivary protein polymorphism, this study was conducted on 160 healthy Koreans between the ages of 20 and 29 chosen randomly
;
their parotid saliva was collected, freeze-dried, and horizontally electrophoresed over acid-urea starch gel. Aluminum lactate-lactic acid was also used as buffer solution. The gel was stained with amidoblack 10B/1% acetic acid solution, and then
destained with 0.5M H2SO4 solution. Accordingly, the parotid middle-band protein(Pm) identified, and its phenotypes and gene frequency were obtained.
@ES The following results were obtained :
@EN 1. The phenotypes of parotid middle band protein(Pm) observed in parotid saliva of the 160 Koreans were Pm(+) in 91 people (56.9%) and Pm(-) in 69 people(43.1%).
2. The gene frequency of Pm+ was 0.343, and that of Pm- waw -.657.
3. The gene frequency of parotid middle band protein(Pm) obtained from Korean's parotid saliva was midway between that of Japanese and Chinese.
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